Bi Botti "Celestin" Youan
Bi Botti "Celestin" Youan, Pharm.D., Ph.D.
Division of Pharmaceutical Sciences
UMKC Health Sciences Building
2464 Charlotte St.
Kansas City, MO 64108
My laboratory research interest is focused on the theoretical and experimental development and evaluation of bioactive agents loaded nanomedicine platforms intended for spatial and temporal controlled release of drug for optimal delivery and targeting to various tissues such as brain, pulmonary, topical routes in the search of better strategy to treat or prevent diseases of high priority in public health. For example, in the first area of novel topical drug delivery system, the work in progress (funded by NIH/NIAID) relates to microbicide loaded biocompatible nanomedicine intended for HIV/AIDS prevention. The second major research area of interest (funded by NIH/NIGMS) is the noninvasive delivery of bioactive macromolecules such as low molecular weight heparin based on the bioengineering of biocompatible microparticles with desirable aerodynamic/general features for controlled release into the alveolar region of the lung. Poor aerosol formulations and delivery systems are the two limitations to improving drug bioavailability through the pulmonary route. Improved particle engineering and various other approaches are being investigated to improve pulmonary bioavailability of bioactive macromolecules. Finally, novel delivery systems and emerging pharmaceutical technologies (e.g. nano/micro-engineering) are also investigated for controlled drug delivery into the brain with potential application to pharmacoresistant epilepsy patients (funding: New Therapy Grant, Epilepsy Research Foundation of America).
The long term research goal is focused on three primary areas: 1) Microbicide loaded nanomedicine for HIV/AIDS prevention 2) Intracerebral Delivery of Anticonvulsants-Loaded Nano/Microparticles, and 3) Heparin Containing Nano/Microparticles for Pulmonary Delivery. Moreover, as indicated below by our group members, we genuinely seek to enhance diversity in biomedical research (aligned with NIH and UMKC missions).
-Microbicide loaded Nanocarrier for HIV/AIDS prevention:
HIV/AIDS remains a major public health concern. There is no efficacious vaccine at this point in time. In this research endeavour is intended to investigate alternative drug delivery methods using nanomedicine platform for topical prevention of the disease transmission. This work is currently funded by NIH/NIAIDS R21 research grant.
-Intracerebral Delivery of Anticonvulsants-Loaded Nano/Microparticles:
This research has been supported by Epilepsy Research Foundation of America (New Therapy Grant). For the first time phenytoin loaded microparticles are engineered and an implantable microdialysis probe is used to deliver anticoagulant and to monitor drug level in the brain in situ. The master formula will be tested for efficacy and safety in kainate induced seizure animal model.
-Heparin Containing Nano/Microparticles for Pulmonary Delivery:
This is another major area of research currently being supported by National Institutes of Health/National Institutes of General Medical Sciences (NIH/NIGMS). The objective of this work is to bioengineer (in a preclinical study) heparin loaded biocompatible microparticles for improved and controlled anticoagulant therapy through the pulmonary route.
Our teaching interest lies mainly in the area of pharmaceutics, the science of dosage forms/drug delivery systems in the undergraduate and graduate programs. Specifically in the undergraduate program, we contribute to UMKC Pharmacy 7202 and Pharmacy 7203 and other related elective courses. The UMKC Pharmacy 7202 course and laboratory relate to the fundamental physicochemical principles basis underlying dosage forms design and performance. The Pharmacy 7203 course relates to the dosage form's composition, manufacturing, evaluation and biopharmaceutical properties for all major routes of drug administration. In the graduate program, our teaching interest focuses on novel drug delivery systems design and the underlying physical pharmacy equilibria and mathematical basis (Phar 5531).
Selected Publications(Since 2007 Only):
Li ZZ, Lee, S Sindee, N Guven, K Borges BB Youan (2007) Formulation of Spray-dried Phenytoin Loaded Poly(epsilon-caprolactone) Microcarrier Intended for Brain Delivery To Treat Epilepsy. Journal of Pharmaceutical Sciences. 96(5):1018-30
Youan, BB. (2008) Impact of nanoscience and nanotechnology on controlled drug delivery. Nanomedicine (Lond). 2008 Aug;3(4):401-6. PMID: 18694301 [PubMed - indexed for MEDLINE]
Bei D, Marszalek J, Youan, BB. Formulation of dacarbazine-loaded cubosomes-part I: influence of formulation variables. AAPS PharmSciTech. 2009;10(3):1032-9. PMID: 19669896 [PubMed - indexed for MEDLINE].
Bei D, Marszalek J, Youan BB. Formulation of dacarbazine-loaded Cubosomes--part II: influence of process parameters. AAPS PharmSciTech. 2009;10(3):1040-7. PMID: 19688599 [PubMed - indexed for MEDLINE].
Youan, BB. (2009) An Introduction to Chronopharmaceutics. In: Youan, BBC (ed). Chronopharmaceutics, The Science and Technology of Biological Rhythm Guided Therapy and Prevention of Diseases. John Wiley & Sons, Hoboken, New Jersey, p 1-30.
Youan, BB.(2010) Chronopharmaceutical drug delivery systems: Hurdles, hype or hope? Adv Drug Deliv Rev. 2010 May 12. [Epub ahead of print] PMID: 20438781 [PubMed - as supplied by publisher].
Endale Abebe Mengesha, Youan, BB (2010) Anticancer Activity and Nutritional Value of Extracts of the Seed of Glinus lotoides. Journal of Nutritional Science and Vitaminology (in press)
Nisha Vijay, James Murowchick, Youan, BB (2010). Thermodynamic Analysis of Phenytoin Encapsulation into Poly (D, L-lactic acid) Nanocarrier. Current Drug Delivery (in press).
Ganti, V, Endale, MA, Marszalek, J., Youan, BB (2010). Statistical analysis of low molecular weight heparin nanoencapsulation. Acta Pharmaceutica (in press).
Zhang, T., Murowchick J., Youan, BB (2010). Optimization of Formulation Variables Affecting Spray dried Oily Core Nanocapsules by Response Surface Methodology. J Pharm Sci (in press).
Bei Di, Tao Zhang, Murrowchick, J. Youan, BB (2010). Formulation of Dacarbazine-Loaded Cubosomes- Part III: Physico-Chemical Characterization. AAPS PharmSci Tech (in press)
Zhang, T., Youan, BB (2010) Analysis of Process Parameters Affecting Spray Dried Oily Core Nanocapsules using Factorial Design. AAPS PharmSci Tech (in press)
Li, ZZ Youan, BB (2010) Imaging Drug Concentration Effect on the Release of Phenytoin From Spray-Dried Poly(-caprolactone) Microcarrier. J. Pharm Sci (in press).
Youan, BB. Chronopharmaceutics: Science and Technology for Biological Rhythm Guided Therapy and Prevention of Diseases. ISBN: 978-0-471-74343-9, 384 pages July 2009.
Poster Abstracts and Presentations at International Conferences (Selected from 2007)
Jwala Gupta, D. Kawatra, D. Pal, BBC Youan (2007). Cytotoxicity assessment of nanodiamonds on mammalian cell lines, The AAPS Journal, Vol. 9, (S2) 2007. Abstract# W4175. http://www.aapsj.org/abstracts/AM_2007/AAPS2007-003587.PDF.
Zhuzhu Li, Youan, BB (2007). Spray dried Porous Structured PLGA-Microcarrier Containing Valproate for Brain Delivery to Treat Epilepsy, The AAPS Journal, Vol. 9 (S2) Abstract# T3238.
N. Vijay, A. M. Endale, Youan, BB (2008). Amphotericin B Loaded Polymersomes for Treatment of Systemic Fungal Infections. 2008 Annual Meeting, Atlanta, Georgia (Accepted). Poster #: W4201. Submission ID: AM-08-02545. http://www.aapsj.org/abstracts/AM_2008/AAPS2008-002545.PDF. AAPS Journal. 2008; 10(S2).
N. Vijay, A. M. Endale, J. Murowchick, Y. Badawi, Youan, BB (2008). Formulation and Characterization of Phenytoin Loaded Poly(lactic acid) Nanoparticles. 2008 Annual Meeting, Atlanta, Georgia. (Accepted). Poster #: T3145. Submission ID: AM-08-01333.http://www.aapsj.org/abstracts/AM_2008/AAPS2008-001333.PDF. AAPS Journal. 2008; 10(S2).
V. Ganti, A. M. Endale, Youan, BB (2008) Formulation and Characterization of Heparin Loaded Nanoparticles for Pulmonary Delivery. 2008 Annual Meeting, Atlanta, Georgia. (Accepted). Submission Poster #: T2109 Submission ID: AM-08-01293. http://www.aapsj.org/abstracts/AM_2008/AAPS2008- 001293.PDF. AAPS Journal. 2008; 10(S2).
D. Bei, J. Murowchick, A. M. Endale, BBC. Youan (2008) Dacarbazine Loaded Cubosome as Potential Nanomedicine for Improved Melanoma Treatment. 2008 Annual Meeting, Atlanta, Georgia. Poster #: T3093. Submission ID: AM-08-01296. http://www.aapsj.org/abstracts/AM_2008/AAPS2008-001296.PDF
X. Yang, A. M. Endale, BBC. Youan (2008) Factors Affecting The Production of Oily Core Nanocapsules. 2008 Annual Meeting, Atlanta, Georgia. (Accepted). 7. Poster #: T3151. Submission ID: AM-08-01968.http://www.aapsj.org/abstracts/AM_2008/AAPS2008-001968.PDF. AAPS Journal. 2008; 10(S2).
Endale, MA, BBC Youan (2008) . Nutritional Value, Antiproliferative and Apoptotic Effects of the Seed of Glinus lotoides. 2008 Annual Meeting, Atlanta, Georgia. (Accepted). Poster #: R6110. Submission ID: AM-08- 01282. http://www.aapsj.org/abstracts/AM_2008/AAPS2008-001282.PDF.
Youan, BBC and Nisha, Vijay. Production of Phenytoin Loaded Polyester Nanoparticles by Spray Drying. AAPS Annual Meeting. (Accepted - Published, 2009 AAPS Annual Meeting, Los Angeles, CA).
Nisha, Vijay; Tao, Zhang; Sarah, Kieweg and Youan, BB (2009);. Assessment of the Viscoelastic Properties of Chitosan Dispersion Intended for Nanoformulations. AAPS Annual meeting. (Accepted - Published2009 AAPS Annual Meeting, Los Angeles, CA).
Zhang T. Youan, BB (2009);. Formulation Variables Influencing Spray Drying Indomethacin Containing Oily Core Nanocapsules. AAPS Annual Meeting. (Accepted - Published, 2009 AAPS Annual Meeting, Los Angeles, CA).
Zhang, T., Youan, BB. (2009) Optimization of Process Parameters for the Production of Indomethacin Containing Oily Core Nanocapsules by Spray Drying. AAPS Annual Meeting. (Accepted , 2009 AAPS Annual Meeting, Los Angeles, CA)
Vivek Agrahari, Youan, BB (2010) Preparation and Characterization of Hyaluronic Acid Based Nanocarriers for Topical Delivery. (Abstract ID: AM-10-04301, Accepted 2010 AAPS Annual Meeting, November, LA).
Zhang, T, Sturgis, T and Youan, BB (2010). Production of Tenofovir Loaded pH-Sensitive Nanoparticle for HIV Prevention (Abstract ID: AM-10-03184, Accepted 2010 AAPS Annual Meeting, November, LA).
J. Meng, Youan, BB (2010). Preparation of Tenofovir Loaded Chitosan Nanoparticles (Abstract ID: AM-10-03910, Accepted 2010 AAPS Annual Meeting, November, LA).
D. Alukda, V. Agrahari, T. F. Sturgis, B-B. C. Youan (2010) Formulation of Microbicide-Loaded Solid Lipid Nanoparticles For Topical Delivery (Abstract ID: AM-10-03161, Accepted 2010 AAPS Annual Meeting, November, LA).
I. Youm, Youan, BB (2010) Preparation of Furosemide Loaded Nanocarrier for Inner Ear Drug Delivery (Abstract ID: AM-10-03190, Accepted 2010 AAPS Annual Meeting, November, LA).
V. Agrahari, Youan, BB (2010) Quantitative Determination of Tenofovir by Reversed Phase High Performance Liquid Chromatography (Abstract ID: AM-10-03170, Accepted 2010 AAPS Annual Meeting, November, LA).